Macrosep® Advance Centrifugal Devices

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Quickly Concentrates Up To
20 mL of Biological Sample

• Rapidly concentrates 20 mL sample volumes to 0.5 mL
• Provides high recoveries, typically > 90%
• Low protein-binding Omega™ membrane and polypropylene housing minimize losses due to non-specific binding
• Versatile Omega membrane is available in a variety of MWCOs
• Built-in deadstop prevents spinning to dryness
• Color-coded for easy identification

Ultrafiltration

• Concentrate and desalt proteins
• Exchange buffer or remove salt of chromatography eluates and gradient fractions
• Recover proteins or other molecules from cell culture supernatants

Microfiltration

• Remove particulate from aqueous solutions and clinical samples

Materials of Construction

• Filter Media: Omega (modified polyethersulfone) and Supor® (polyethersulfone) membranes
• Sample Reservoir, Filtrate Receiver, and Cap: Polypropylene
• Paddle: Polyethylene

Effective Filtration Area

• 7.2 cm²

Dimensions

• Diameter: 29 mm (1.2 in.)
• Length: 12.0 cm (4.7 in.)

Operating Temperature Range

• 0 - 40 °C (32 - 104 °F)

Capacities

• Maximum Sample Volume: 20 mL
• Final Concentrate Volume:
     450 μL (swinging bucket)
     1.2 - 1.5 mL (45º angle rotor)
     1.5 mL (34º angle rotor)
• Filtrate Receiver Volume: 22 mL
• Hold-Up Volume: 80 μL (membrane and paddle)

pH Range

• 1 - 14

Maximum Centrifugal Force

• 5,000 x g (ultrafiltration)
• 14,000 x g (microfiltration)

Centrifuge

• Fits centrifuges that accept standard 50 mL conical end tubes

Sanitization

• Provided non-sterile. May be sanitized by filtering 70% ethanol through thedevice prior to use.

Macrosep Advance Centrifugal Devices: Reduced Spin Time

Protein solutions were processed in each of the Macrosep Advance devices. Average time (minutes) is plotted against mL of remaining product to be filtered using a swinging bucket rotor at 5,000 x g. Solutions are 3K: Protamine Sulfate, 0.1% in 1X PBS; 10K: Cytochrome C, 0.025% in 1X PBS; 30K: IgG, 0.1% in 1X PBS; and 100K: Apoferritin, 0.1% in 1X PBS.

Macrosep Advance Centrifugal Devices: Retention Efficiency

Protein solutions were processed in each of the Macrosep Advance devices. Average percent retention using a swinging bucket rotor at 5,000 x g is displayed for each MWCO. Solutions were 3K: Protamine Sulfate, 0.1% 1X PBS; 10K: Cytochrome C, 0.025% in 1X PBS; 30K: IgG, 0.1% in 1X PBS; and 100K: Apoferritin, 0.1% in 1X PBS.