HEA and PPA HyperCel™ Mixed-mode Chromatography Sorbents

For Selectivities Not Accessible with
Traditional Ion Exchange or HIC

• Chromatographic behavior “mixed-mode” based on a combination of electrostatic and hydrophobic properties of the protein and ligands
• Provides direct hydrophobic capture of proteins at low ionic strength
• Can be exploited to achieve discrimination of proteins having similar or very close isoelectric points, a separation that cannot be performed by methods like ion exchange
• Can be used in a “physiological-like” environment (no need for pH adjustment of the feedstream; no, or limited, addition of salt)
• Orthogonal to ion exchange or other chromatography steps

• Fractionation of a protein mix
• Separation of partially-purified polyclonal IgG from major plasma impurities
• Process polishing steps

Particle Size

• 80 - 100 µm (average)

Bead Composition

• High porosity cross-linked cellulose

Dynamic Binding Capacity for BSA (10% breakthrough)¹

• 40 - 60 mg/mL

Ligand

• Aliphatic (HEA): Hexylamine
• Aromatic (PPA): Phenylpropylamine

Adsorption pH

• 7 - 9

Elution pH

• By gradient or step elution, e.g. 7.0 - 2.6

Working pH

• 2 - 12

Pressure Resistance

• < 3 bar (300 kPa, 44 psi)

Storage Temperature

• 2 - 8 °C (36 - 46 °F)

¹Determined using 5 mg/mL BSA in PBS; flow rate: 100 cm/h

AcroSep™ Chromatography Columns for Mixed-Mode

Comparison Between HEA and PPA HyperCel, MEP HyperCel, Conventional HIC, and Anion Exchange Sorbents 

Adsorption/desorption of Bovine Serum Albumin (BSA), in PBS buffer, pH 7.4. Data shows that BSA is efficiently retained on both HEA and PPA HyperCel sorbents, but is poorly retained on MEP HyperCel sorbent (as this ligand is antibody-selective). In PBS, without lyotropic salt addition, low binding of BSA to both Phenyl and Hexyl HIC sorbents is observed. The anion exchange (DEAE) sorbent also did not bind BSA at these nonoptimal conditions (pH 7.4 and too high salt concentration).

Part Number	Description	Pkg	Price	Qty
AcroSep Chromatography Columns for Mixed-Mode
20260-C001	PPA HyperCel, 1 mL, Yellow	5/pkg	111.72
20250-C001	HEA HyperCel, 1 mL, Black	5/pkg	111.72
HEA HyperCel Mixed-mode Chromatography Resin
20250-012	HEA HyperCel	5 mL	72.80
20250-026	HEA HyperCel	25 mL	119.60
20250-033	HEA HyperCel	100 mL	452.40
20250-041	HEA HyperCel	1000 mL	3,212.56
PPA HyperCel Mixed-mode Chromatography Resin
20260-015	PPA HyperCel	5 mL	72.80
20260-025	PPA HyperCel	25 mL	119.60
20260-030	PPA HyperCel	100 mL	452.40
20260-040	PPA HyperCel	1000 mL	3,212.56

Contact Customer Service at 800-521-1520 for possible lead-time and ship date.