AcroSep™ Chromatography Columns for Affinity Purification
Print Product Information
E-Mail
Download PDF
For the Purification of a Wide
Variety of Proteins and Antibodies
Protein A Ceramic HyperD® F Sorbent
- Achieves > 90% recovery of IgG in a single step
- Recombinant Protein A immobilized to specially formulated hydrogel within a ceramic bead offers high selectivity with low non-specific binding and low levels of Protein A leaching
IMAC HyperCel™ Sorbent
- Provides single-step purity under both native and denaturing conditions
- IMAC HyperCel sorbent uses tridentate IDA (iminodiacetic acid) chelating ligand immobilized on the HyperCel bead resulting in a stable and robust sorbent for small-to-large scale protein separation
- Flexible, uncharged IMAC HyperCel sorbent offers the option to select specific metal ions to achieve high purity and yield of target proteins
Blue Trisacryl® M Sorbent
- Trisacryl macroporous base matrix allows good diffusion and improves exchange kinetics
- Cibacron♦ blue dye ligand selective for enzymes and albumin
- Enhanced stability due to ligand coupling mechanism prevents dye leakage
Protein A Ceramic HyperD F Sorbent
- Designed for IgG affinity purification from multiple sample types
- Purification of Fc fusion and Ab-like proteins
- Depletion of IgG from plasma and serum
IMAC HyperCel Sorbent
- Ideal for purification of His-tagged proteins
- Purification of metal binding proteins or other proteins capable of metal ion coordination
Blue Trisacryl M Sorbent
- Optimized for depletion of albumin from plasma and serum samples
- Good for purification of some enzymes
Materials of Construction
- Column Housing, Cap, Plug, and Adapter: Polypropylene
- Column Frit: Polyethylene
| Media | Color | Average Particle Size | Working pH | Capacity |
| Protein A Ceramic HyperD F | Pearl | 50 µm | 2 - 11 | > 30 mg/mL1 |
| IMAC HyperCel | Cobalt blue | 90 µm | 3 - 12 | 40 - 70 μmol Cu2+/mL |
| Blue Trisacryl M | Dark blue | 60 µm | 1 - 10 | 10 - 15 mg/mL2 |
Column Geometry
- Column Volume: 1.04 mL
- Bed Height: 1.48 cm (0.58 in.)
- Bed Diameter: 0.94 cm (0.37 in.)
Device Dimensions
- Diameter: 1.6 cm (0.6 in.)
- Length (Without Plugs): 4.8 cm (1.9 in.)
Connections
- Inlet: Threaded female luer lock
- Outlet: Rotating male luer locking hub
Recommended Flow Rates
- Protein A Ceramic HyperD F: 0.2 - 1 mL/min
- IMAC-Metal Loading Step: 3 - 6 mL/min
- IMAC-Protein Capture Step: 0.5 - 1 mL/min
- Blue Trisacryl M: 0.2 - 1.0 mL/min
Back Pressure
- Maximum: 3 bar (300 kPa, 43.5 psi)
Storage
- 2 - 8 ºC (36 - 46 ºC)
110% breakthrough, 100 cm/hr, determined using 10 mg/mL human IgG in PBS, pH 7.4, elution in 0.1 M sodium citrate, pH 2.5, column 4.6 ID x 100 mm.
2Binding capacity using 5 mg/mL human albumin in PBS. Column dimensions – 1.6 cm ID x 3 cm bed height; residence time (Tr) = 7.26 min.
- Blue Trisacryl M Chromatography Sorbent
- IMAC HyperCel Chromatography Sorbent
- Protein A Ceramic HyperD F Chromatography Sorbent
-
View additional chromatography products and technology.
AcroSep™ Columns Provide Greater Capacity of IgG Compared to Competitive Columns
Recovery of IgG from human plasma quantified by ELISA. Data shows higher recovery of IgG with AcroSep Protein A Ceramic HyperD® F columns as compared to competitive columns. Average value is derived from duplicate readings. (Flow rate: 0.2 mL/min for loading, 1 mL/min all other steps.)
High Purity Protein in a Single Step Using Native Conditions
M: Protein molecular weight marker
L: Sample loaded on the column
FT: Unbound/flow through W: Wash fraction
E: Eluate
Marked area on gel demonstrates single-step affinity purification of His-tagged protein purified under native conditions on IMAC HyperCel™.
Purification of Albumin From Human Plasma
Total protein (0.3 mL plasma) was bound to the Blue Trisacryl® M AcroSep column in 20 mM PBS, pH 7.2. Bound proteins were eluted with a gradient of 3 M NaCl in PBS.
High Purity Protein in a Single Step Using Native Conditions
M: Protein molecular weight marker
L: Sample loaded on the column
FT: Unbound/flow through
W: Wash fraction
E: Eluate
Marked area on gel demonstrates single-step affinity purification of His-tagged protein purified under denaturing conditions on IMAC HyperCel.
Contact Customer Service at 800-521-1520 for possible lead-time and ship date.
|
Your shopping cart is empty. |
| *US List Price applies only to products delivered within the US. These prices do not reflect duties, taxes or tariffs, which may apply to purchases made outside of the US. See our General Limited Warranty. |