AcroSep™ Chromatography Columns for Ion Exchange

Rapid, High Capacity Protein Purification
in a User-Friendly Format

• Patented ceramic HyperD® ion exchange chromatography sorbent features “gel-in-a-shell” technology, providing rapid protein purification with high capacity and good resolution.
• Rapid and efficient. Obtains high speed runs (1 - 4 mL/min) with very little loss in binding capacity and resolution, allowing more samples to be processed per day.
• Higher resolution for 1 mL columns. Columns provide distinct separation of proteins for better purification.
• Luer lock inlet and outlet allows convenient use with syringe, pump, or automated chromatography system.
• User-friendly column design is colorcoded and labeled by chemistry type. Collar is hexagonal so columns will not unexpectedly roll off lab surface.

• Screen multiple IEX chemistries for use in protein purification
• Ideal for optimization studies of protein purification schemes using small sample volumes prior to scale-up
• Efficient and reliable small scale purification of proteins for structural, functional, and yield analysis

Materials of Construction

• Column Housing, Cap, Plug, and Adapter: Polypropylene
• Column Frit: Polyethylene

Media	Function	Color Code	Particle Size	Working pH	Ion Exchange Capacity1
CM Ceramic HyperD F	Weak cation exchanger	Green	50 μm (avg)	2 - 12	> 60 mg/mL2
DEAE Ceramic HyperD F	Weak anion exchanger	Orange	50 μm (avg)	2 - 12	> 85 mg/mL3
Q Ceramic HyperD F	Strong anion exchanger	Red	50 μm (avg)	2 - 12	> 85 mg/mL3
S Ceramic HyperD F	Strong cation exchanger	Clue	50 μm (avg)	2 - 12	> 75 mg/mL4

Column Geometry

• Column Volume: 1.04 mL
• Bed Height: 1.48 cm (0.58 in.)
• Bed Diameter: 0.94 cm (0.37 in.)

Device Dimensions

• Diameter: 1.6 cm (0.6 in.)
• Length (Without Plugs): 4.8 cm (1.9 in.)

Connections

• Inlet: Threaded female luer lock
• Outlet: Rotating male luer locking hub

Recommended Flow Rates

• 1 - 4 mL/min

Back Pressure

• Maximum: 3 bar (300 kPa, 43.5 psi)

Storage

• 2-30 ºC (36-86 ºF)
• 2-8 ºC (36-46 ºF) after opening

¹Dynamic binding capacity (DBC) determined at 10% breakthrough, 200 cm/h with 1.66 mL sorbent packed in a column of 5 mm ID and 100 mm height using the following:
²5 mg/mL human IgG in 50 mM sodium acetate buffer, 100 mM NaCl, pH 4.7
³5 mg/mL BSA in 50 mM Tris-HCl buffer, pH 8.6
⁴5 mg/mL lysozyme in 50 mM sodium acetate buffer, pH 4.5

Instructions for Use: AcroSep™ Columns for Ion Exchange Chromatography

Resolution Comparison of AcroSep™ Columns With Q HyperD® F and Competitive Q at Flow Rate of 1 mL/min

Resolution was calculated using default algorithm on AKTA^♦ chromatography system.

The chromatogram above is an illustration of resolution between BSA and Conalbumin using three replicates of Pall's AcroSep Q column and a competitive Q column. This separation was run at 1 mL/min. Resolution of the AcroSep columns is clearly illustrated in this chromatogram. The competitive column is represented by the green line.

Resolution Comparison of AcroSep Columns With Q HyperD F and Competitive Q at Flow Rate of 4 mL/min

The above chromatogram is an illustration of the resolution of the AcroSep Q column and the competitive column when the flow rate is elevated to 4 mL/min. It demonstrates that at 4 mL/min there is little, if any, change in protein separation on the AcroSep column, while the competitor performance degrades significantly with increased flow rate.

1 mL AcroSep Column Average Dynamic Binding Capacity at High Flow Rate (10% Breakthrough)

The above illustrates dynamic binding capacity for each of our four ion exchange chemistries when run at 3.56 mL/min. Data shows higher dynamic binding capacities of Pall AcroSep columns over competitive columns at higher flow rates. Each average is derived from three replicates of > 8 AcroSep columns or three replicates of two competitive columns. The proteins were at a concentration of 5 mg/mL and the choices were BSA for Q and DEAE, Lysozyme for S, and IgG for CM.

Part Number	Description	Pkg	Price	Qty
AcroSep Chromatography Columns for Ion Exchange
20050-C001	CM Ceramic HyperD F, 1 mL, Green	5/pkg	122.72
20062-C001	S Ceramic HyperD F, 1 mL, Blue	5/pkg	122.72
20066-C001	Q Ceramic HyperD F, 1 mL, Red	5/pkg	122.72
20067-C001	DEAE Ceramic HyperD F, 1 mL, Orange	5/pkg	122.72
IEXVP-C001	(1) each: Q, S, CM, and DEAE Ceramic HyperD F, 1 mL	4/pkg	122.74

Contact Customer Service at 800-521-1520 for possible lead-time and ship date.