IMAC HyperCel™ Sorbent for Immobilized Metal Affinity Chromatography (IMAC)

Ideal for Tagged Biomolecule
Purification Using IMAC

• Metal-chelate affinity chromatography exploits the affinity of proteins or other molecules for metal ions. 
• Can be used in combination with standard chromatography columns, disposable gravity flow columns, disposable Nanosep® centrifugal filters and AcroPrep™ multi-well filter plates. 
• Uses tridentate IDA (iminodiacetic acid) as a chelating ligand agent. This ligand is immobilized on the HyperCel base sorbent, a stable, robust and well-known sorbent used for both research and industrial-scale protein separations.
• Prior to use, metal ions can be immobilized on the IMAC HyperCel resin.

• Purification of His-tagged proteins
• Purification of phosphoproteins and phosphopeptides
• Purification of antibodies
• Fractionation of complex protein mixtures

Particle Size

• 80-100 µm

Typical Working Pressure

• < 1 bar (100 kPa, 14 psi)

Capacity for Metal Ions

• 40-70 µmol Cu² per mL of sorbent

Ionic Capacity

• 90-140 µeq/mL

Regeneration

• 0.5-1.0 M NaOH

Pressure Resistance

• < 3 bar (300 kPa, 44 psi)

Working pH

• 3 - 12

Storage Temperature

• 2-8 °C (36-46 °F)

• AcroSep™ Chromatography Columns for Affinity Purification

High Purity Protein in a Single Step Using Native Conditions

M: Protein molecular weight marker
L: Sample loaded on the column
FT: Unbound/flow through
W: Wash fraction
E: Eluate

Marked area on gel demonstrates single-step affinity purification of His-tagged protein purified under native conditions.

High Purity Protein in a Single Step Using Denaturing Conditions

M: Protein molecular weight marker
L: Sample loaded on the column
FT: Unbound/flow through
W: Wash fraction
E: Eluate

Marked area on gel demonstrates single-step affinity purification of His-tagged protein purified under denaturing conditions.

Part Number	Description	Pkg	Price	Qty
IMAC HyperCel Affinity Chromatography Resin
20093-069	IMAC HyperCel	5 mL	62.40
20093-010	IMAC HyperCel	25 mL	196.56
20093-028	IMAC HyperCel	100 mL	582.40
20093-036	IMAC HyperCel	1000 mL	5,418.40

Contact Customer Service at 800-521-1520 for possible lead-time and ship date.