SDR HyperD® Detergent Removal Chromatography Sorbent

Unique Sorbent Designed to Eliminate Detergent from Biological Fluids

• High dynamic binding capacity for many different detergents.
• High recovery for proteins.
• High adsorption capacity for small, hydrophobic molecules.
• Stable in acid, polar organic, and oxidizing solutions.
• Composed of silica beads in which the pore volume is filled with a three-dimensional crosslinked hydrophobic polymer. The small pore size, particle size distribution (40 to 100 μm), and hydrophobic nature of the chemical groups make SDR HyperD sorbent ideal for detergent removal.

• Binds detergent molecules typically used in viral inactivation processes [i.e., Tri-n-Butyl Phosphate (TnBP) and Tritons X-100]
• Removal of both ionic and zwitter ionic detergents from biological solutions

Particle Size

• 40 - 100 μm

Nature of Polymer

• Hydrophobic, long aliphatic chains bind solvents

Recommended Residence Time

• 5 - 15 min

Binding Capacity for Triton X-100

• 60 - 80 mg/mL¹

Working pH

• 2 - 12

Pressure Resistance

• 70 bar (7,000 kPa, 1,000 psi)

Storage Temperature

• 2 - 30 ºC (36 - 86 ºF)
• 2 - 8 ºC (36 - 46 ºF) after opening
• Do not freeze

¹Determined using 5 mg/mL Triton X-100 in PBS, pH 7.4, 10% breakthrough, 300 cm/h.

• Q, S, DEAE, CM Ceramic HyperD® Ion Exchange Sorbents, Laboratory Scale Volumes
• Nanosep® & Nanosep MF Centrifugal Devices
• Nanosep® MF Centrifugal Devices with GHP Membrane 
• AcroPrep™ 384 Filter Plates, 100 µL, for Sample Preparation and Detection
• AcroPrep™ 96 Filter Plates, 1 mL, for Sample Preparation and Detection
• AcroPrep™ 96 Filter Plates, 350 µL, for Sample Preparation and Detection
• AcroWell™ 96 Membrane-bottom Plates with Binding Membranes for Detection and Screening
• AcroWell™ 96 Membrane-bottom Plates with GHP Membrane for Detection and Screening
• Protocols for SDR HyperD Solvent-Detergent Removal Chromatography Resins
• PII: SDR HyperD® Solvent-Detergent Removal Sorbent

SDR HyperD Detergent Removal: Spin Column Format (< 0.2 mL Volume)

Detergent	Removal Efficiency from a Protein Sample1
Volume (Wt./v)	+ 1%	+ 5%	+ 10%
ASB-14	> 99%	> 99%	80-90%
ASB-14 + 6M urea	> 99%	80-90%	NR
CHAPS	> 99%	> 99%	> 99%
CHAPS + 6M urea	> 99%	> 99%	80-90%
SDS	> 99%	> 99%	> 99%
SDS + 0.1 M NaCl	> 99%	> 99%	> 99%

¹10.2 mL of 5 mg/mL BSA in the presence of detergents (Wt./v). Bio-Rad dye binding assay used to follow removal of detergents. NR denotes “Not Recommended” as this detergent loading would exceed the column capacity.

Using a rapid spin column format, the binding capacity of SDR for detergents is still very high. In all cases, > 99% of detergent removed from 0.2 mL of a 1% solution.

Part Number	Description	Pkg	Price	Qty
AcroSep Chromatography Columns for Mixed-Mode
20033-C001	SDR HyperD, 1 mL, Natural	5/pkg	176.53
SDR HyperD Detergent Removal Chromatography Sorbent
20033-065	SDR HyperD	5 mL	65.52
20033-031	SDR HyperD	25 mL	109.20
20033-023	SDR HyperD	100 mL	322.40

Contact Customer Service at 800-521-1520 for possible lead-time and ship date.