AcroWell™ 96-well Membrane-bottom Plates with BioTrace™ PVDF and NT Membranes

Excellent for Parallel or Automated
DNA and Protein Detection Applications

• Plate and membrane construction allows long incubations. Dual membrane construction allows support and protection of upstream layer. Downstream membrane also acts as a barrier to passive flow.
• Black, white, and natural housing for fluorescent, luminescent or radioactive detection systems.
• Robotic-friendly design has rigid single-piece construction. Plates are stackable with or without lids.
• Membrane is sealed to bottom of plate using proprietary sealing technology that eliminates crosstalk.
• A serialized barcode label allows the use of automated tracking systems and identifies the membrane type.
• Polypropylene filter plate housing is chemically resistant and low in biomolecule binding.

Compatible with Manual Filtration or with Robotic Handling Systems

BioTrace NT Membrane

• Protein dot blots
• Nucleic acid dot blots
• Solid phase ELISA
• Drug discovery using bound molecules

BioTrace PVDF Membrane

• Protein:nucleic acid interaction studies
• Protein dot blots
• ELISPOT
• Kinase assays
• ELISA

Materials of Construction

• Filter Media: BioTrace NT (nitrocellulose) and BioTrace PVDF (polyvinylidene fluoride) membranes
• Membrane Support: Emflon® membrane (PTFE) backed with nonwoven polypropylene
• Plate Housing: Polypropylene (natural, black, or white opaque)
• Lid: Polystyrene

Dimensions

• Length: 12.8 cm (5.0 in.) Width: 8.6 cm (3.4 in.) Height With Lid: 1.7 cm (0.7 in.) Height Without Lid: 1.4 cm (0.6 in.)

Well-Bottom Area

• 0.3 cm2

Maximum Well Volume

• 350 μL

Recommended Working Volume

• 250 μL

Recommended Operating Vacuum

• 25.4 cm Hg (10.0 in. Hg)

Typical Hold-Up Volume

• < 10 μL/well

AcroPrep 384-Well Filter Plates

Binding of Antibodies to Various Membranes 

Dilutions of Cy5-labeled antibodies were added to wells that were either pre-wet (PVDF) or dry (NT and GHP). Antibody dilutions ranged from 1000 ng to less than 34 ng. Aliquots of 200 μL of each dilution were added, incubated for five minutes at room temperature, and filtered at 25.4 cm Hg (10 in. Hg) vacuum. A total of three 200 μL PBS washes were followed by detection in a Victors multilabel counter (PerkinElmer, Inc.). GHP was used as a low binding control. Error bars indicate standard deviation, n = 8. Second graph shows the logarithm plots for the BioTrace™ NT and BioTrace PVDF membrane-containing plates. CPS = Counts Per Second.

Part Number	Description	Pkg	Price	Qty
AcroWell™ 96-well Filter Plates with BioTrace NT Membrane
5022	0.2 µm, BioTrace NT membrane, white	10/pkg	155.86
5025	0.2 µm, BioTrace NT membrane, black	10/pkg	155.86
AcroWell 96-well Filter Plates with BioTrace PVDF Membrane
5023	0.45 µm, BioTrace PVDF membrane, natural	10/pkg	155.86
5026	0.45 µm, BioTrace PVDF membrane, black	10/pkg	155.86
5027	0.45 µm, BioTrace PVDF membrane, white	10/pkg	155.86
Accessories and Replacement Parts
5017	Multi-well plate manifold	1/pkg	1,087.25
5225	Adapter collar for centrifugation	2/pkg	80.88
5230	Cap mat for incubation	5/pkg	44.53
5231	Multi-well plate lids	10/pkg	36.34

Contact Customer Service at 800-521-1520 for possible lead-time and ship date.