HA Ultrogel® Hydroxyapatite Chromatography Sorbent

For the Separation of Proteins,
Peptides, and Nucleic Acids

• High binding capacity and high flow rates
• Structure provides excellent rigidity and stability to pH and ionic strength changes, as well as to high temperature
• Easy cleaning with sodium hydroxide
• Scalable from research and development to manufacturing

• Separation of basic proteins (Cytochrome C, Lysozyme, etc.)
• Separtaion of human serum proteins and plant proteins such as lectins, glycoproteins, transferases, kinases, and trehalases
• Separation of phosphate dependent proteins and exzymes as well as
  DNA-dependent enzymes
• Can be used to remove DNA and endotoxins

Particle Size

• 60 - 180 µm

Hydroxyapatite Content (Weight/Volume)

• 40%

Agarose (Weight/Volume)

• 4%

Exclusion Limit

• > 5,000,000 Da

Capacity for Cytochrome C¹

• > 7 mg/mL

Capacity for BSA²

• < 7 mg/mL

Working pH

• 5 - 13 (Note: Do not use < pH 4.0.)

Storage Temperature

• 2 - 30 ºC (36 - 86 °F)
• 2 - 8 ºC (36 - 46 °F) after opening

¹Determined at 50% breakthrough using 5 mg/mL Cytochrome C diluted 50/50 in 10 mM PBS, pH 6.8
at 30 cm/h.
²Determined at 50% breakthrough using 1 mg/mL BSA diluted 50/50 in 10 mM PBS, pH 6.8 at 12.5 cm/h.

Separation of a Mixture of Ribonuclease and Phytohemagglutinins (PHA-EL)

Column: 1.6 x 6.5 cm; Sample: 1 mg of protein mixture composed of ribonuclease (MW 14,700) and PHA-ELs (Erythroagglutinating and lymphostimulating Phytohemagglutinin) (MW 128,000) from Phaseolus vulgaris, in 1 mL of 5 mM potassium phophate, pH 6.8; Elution gradient: 5 mM to 500 mM potassium phosphate, pH 6.8; Flow rate: 14.4 cm/h.

Separation of Trypsin and Chymotrypsin from a Porcine Pancreatic Enzyme Extract

Column: 1.6 x 5 cm; Sample: 30 mg protein in 1 mL of 5 mM phosphate buffer, pH 6.8; Gradient: 5 to 200 mM sodium phosphate, ph 6.8; Flow rate: 10cm/h; Temperature: 10 °C; Histogram with broken line: trypsiin activity; Histogram with solid line: chymotrypsin activity. Spec. Ac.: specific activity in U/mg. tr: trypsin, ch: chymostrypsin. Trypsin activity was primarily found in the peak eluted by 50 mM phosphate where the chymotrypsin was eluted by 100 mM phosphate. The final yilad was approximately 50%.

Part Number	Description	Pkg	Price	Qty
HA Ultrogel® Hydroxyapatite Chromatography Sorbent, Laboratory Scale Volumes
24775-075	HA Ultrogel Hydroxyapatite	5 mL	62.40
24775-082	HA Ultrogel Hydroxyapatite	25 mL	113.36
24775-025	HA Ultrogel Hydroxyapatite	100 mL	239.20
24775-017	HA Ultrogel Hydroxyapatite	500 mL	1,019.20

Contact Customer Service at 800-521-1520 for possible lead-time and ship date.