MEP HyperCel™ Mixed-Mode Chromatography Sorbent

For the Capture and Purification of
Monoclonal and Polyclonal Antibodies

• High purity in a single step - Product purities of 70% to 90% or greater are typically achieved
• Rapid sample processing - Dynamic binding capacities of > 30 mg IgG per mL of sorbent (at 10% breakthrough) are routinely achieved
• Exhibits high IgG capacity, independent of sub-class or species
• Allows direct sample loading without adjustment of pH or ionic strength
• Effective capture, even with feedstocks as dilute as 50 to 100 μg IgG/mL
• Offers easy cleaning with sodium hydroxide

• Capture and purification of antibodies from a broad range of sources such as animal sera, ascites fluid, and cell culture supernatant.
• Independent of subclass or species. Weakly-binding variants (e.g., murine IgG, rat IgG) are well retained.
• Alternative to traditional HIC sorbents for capture of hydrophobic molecules.
• Has been studied in a broad range of applications including isolation of antibodies from sweet-whey and colostrum; isolation of antibodies from transgenic plant and animal sources; and, isolation of IgA and selected fusion proteins

Particle Size

• 80 - 100 µm (average)

Bead Composition

• High porosity cross-linked cellulose

Dynamic Binding Capacity for Human IgG (10% breakthrough)¹

• > 20 mg/mL

Ligand

• 4-Mercaptoethylpyridine (4-MEP)

Ligand Density

• 80 - 125 µmol/mL

Working pH

• 2 - 12

Pressure Resistance

• < 3 bar (300 kPa, 44 psi)

Storage Temperature

• 2 - 8 °C (36 - 46 °F)

¹Determined using 5 mg/mL human IgG in PBS, flow rate 60 cm/h.

AcroSep™ Chromatography Columns for Mixed-Mode 

Monoclonal Antibodies Purification on MEP
HyperCel Resin from Cell Culture Supernatants

Panel A: Serum-free Cell Culture Media 

Panel B: 5% Serum-supplemented Cell Culture Media

Sample A = 300 mL protein-free cell culture supernatant. Sample B = 300 mL cell culture supernatant containing 5% fetal bovine serum; Equilibration: 50 mM Tris-HCI, pH 8; Elution: 50 mM acetate, pH4; Flow rate: 70 cm/h. In curve B, (a) and (b) are respectively water and 25 mM sodium caprylate washings; SDS-PAGE (reduced conditions): (1) = crude sample; (2) = purified IgG; (H) = heavy chain; (L) = light chain.

Part Number	Description	Pkg	Price	Qty
AcroSep™ Chromatography Columns for Mixed-Mode
12035-C001	MEP HyperCel, 1 mL, Purple	5/pkg	154.47
MEP HyperCel Hydrophobic Charge Induction Chromatography (HCIC) Resin
12035-069	MEP HyperCel	5 mL	72.80
12035-010	MEP HyperCel	25 mL	119.60
12035-028	MEP HyperCel	100 mL	452.40

Contact Customer Service at 800-521-1520 for possible lead-time and ship date.