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Heparin HyperD® M Affinity Chromatography Sorbent

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For the Purification of Biological
Molecules that Bind to Heparin

Design combines the desirable characteristics of a soft, high speed, high capacity hydrogel with the high dimensional stability of a rigid bead
Leakage is minimized due to the stable chemical link of the heparin molecule to the sorbent
Rapid packing due to the high density of the bead
Can be packed in a variety of column sizes


Part Number	Description	Pkg	Price	Qty
Heparin HyperD® M Affinity Chromatography Resin
20029-062	Heparin HyperD M	5 mL	104.00
20029-039	Heparin HyperD M	25 mL	213.20
20029-021	Heparin HyperD M	100 mL	613.60

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*US List Price applies only to products delivered within the US. These prices do not reflect duties, taxes or tariffs, which may apply to purchases made outside of the US. See our General Limited Warranty.

Heparin HyperD® M Affinity Chromatography Sorbent

For the Purification of Biological
Molecules that Bind to Heparin

Particle Size

Dynamic Binding Capacity for Human ATIII (10% Breakthrough)¹

Ligand

Immobilized Heparin/mL Sorbent

Working pH

Pressure Resistance

Storage Temperature

¹Determined using human ATIII at 72.5 UI/mL in 20 mM TrisHCl, 0.3 M NaCl, pH 7.4. Elution with 20 mM Tris HCl, 2 M NaCl, pH 7.4 at a flow rate of 600 cm/h, 10 cm (4 in.) bed height.

Dynamic Binding Capacity vs. Linear Velocity

Column dimensions: 0.46 cm I.D. x 10 cm; Sample: HU ATIII at 72.5 μL/mL; Equilibration buffer: 20 mM Tris-HCl containing 0.3 M NaCl, pH 7.4.

Pressure vs. Linear Flow Velocity

Column dimensions: 0.46 cm I.D. x 10 cm; Buffer: 20 mM Tris-HCl containing 0.3 M NaCl, pH 7.4.

Heparin HyperD® M Affinity Chromatography Sorbent

For the Purification of Biological Molecules that Bind to Heparin

Particle Size

Dynamic Binding Capacity for Human ATIII (10% Breakthrough)1

Ligand

Immobilized Heparin/mL Sorbent

Working pH

Pressure Resistance

Storage Temperature

1Determined using human ATIII at 72.5 UI/mL in 20 mM TrisHCl, 0.3 M NaCl, pH 7.4. Elution with 20 mM Tris HCl, 2 M NaCl, pH 7.4 at a flow rate of 600 cm/h, 10 cm (4 in.) bed height.

Dynamic Binding Capacity vs. Linear Velocity

Column dimensions: 0.46 cm I.D. x 10 cm; Sample: HU ATIII at 72.5 μL/mL; Equilibration buffer: 20 mM Tris-HCl containing 0.3 M NaCl, pH 7.4.

Pressure vs. Linear Flow Velocity

Column dimensions: 0.46 cm I.D. x 10 cm; Buffer: 20 mM Tris-HCl containing 0.3 M NaCl, pH 7.4.

For the Purification of Biological
Molecules that Bind to Heparin

Dynamic Binding Capacity for Human ATIII (10% Breakthrough)¹

¹Determined using human ATIII at 72.5 UI/mL in 20 mM TrisHCl, 0.3 M NaCl, pH 7.4. Elution with 20 mM Tris HCl, 2 M NaCl, pH 7.4 at a flow rate of 600 cm/h, 10 cm (4 in.) bed height.