Blue Trisacryl® M Affinity Chromatography Sorbent
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For the Purification of a Wide
Variety of Enzymes and Proteins
- Strong bond of dye-to-sorbent prevents leakage of the dye in normal working conditions
- Excellent chemical stability is attributed to the synthetic nature of Trisacryl sorbent and the enhanced stability of the ligand coupling mechanism
- Excellent mechanical stability allows high flow rates with working pressures up to 3 bar (300 kPa, 45 psi)
- Basic matrix is Trisacryl GF2000, a macroporous non-ionic sorbent. Cibacron♦ Blue F3GA dye is strongly bound to the matrix through a six-carbon spacer arm
- Albumin removal increases the resolution of both 1D and 2D electrophoresis
- Designed for the purification of a wide variety of enzymes and proteins such as kinases, interferons, and some coagulation factors
- The interaction mechanism between Cibacron Blue F3GA and proteins involves one or more of the following: stereospecific recognition of NAD analogs; electrostatic and hydrophobic interaction; and/or electron exchange
Particle Size
- 40 - 80 µm
Exclusion Limit
- 107 Da
Capacity for Human Albumin1
- 10 - 15 mg/mL
Capacity for Bovine Albumin1
- 5 - 7 mg/mL
Working pH
- 1 - 10
Pressure Resistance
- Up to 3 bar (300 kPa, 44 psi)
Storage Temperature
- 2 - 8 °C (36 - 46 °F)
1Capacity determined in phosphate buffer saline (PBS) using 5 mg/mL.
Analytical Separation of Human Plasma Proteins on Blue Trisacryl M Affinity Chromatography Sorbent
Column: 1.6 cm I.D. x 10 cm; Buffer: 0.05 M Tris-HCI, pH 8.8; Elution performed by a continuous sodium chloride gradient from 0 to 3 M; Flow rate: 100 cm/h; Separation time: 180 min; Temperature: 20 °C.
Contact Customer Service at 800-521-1520 for possible lead-time and ship date.
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