UltraBind™ Affinity Membrane

Modified polyethersulfone (PES) membrane for covalent protein binding

• Proteins can be efficiently attached without prior membrane derivatization.

• ELISA
• Affinity separation

Media

Modified polyethersulfone with aldehyde surface chemistry 

Pore Size

0.45 µm

Typical Thickness

152 µm (6 mils)

Typical IgG Binding Capacity

135 µg/cm²

• Nanosep® & Nanosep MF Centrifugal Devices
• Stainless Steel Forceps

UltraBind Membrane Binds and Retains Proteins

Membrane discs (13 mm) were soaked in a protein solution and washed to determine the capacity and strength of protein binding. Discs were soaked in radioactively-labeled IgG and BSA (200 µg unlabeled protein with 100,000 cpm of ¹²⁵I-labeled tracer) for 60 minutes with agitation, rinsed, and either read in a scintillation counter or stripped using a 1% SDS/2 M Urea wash. Biodyne B membrane (charged nylon transfer membrane) and Bio-Inert membrane (modified Nylon 6,6 membrane) were used as high and low binding capacity controls respectively. UltraBind membrane efficiently bound protein and retained it after the SDS/Urea wash. 

Antigen Detection (dot blot ELISA) with UltraBind Membrane

Dilutions of human serum albumin (hSA) ranging 2000 to 0.5 pg/spot were applied to UltraBind membrane using a 96-pin transfer tool on a Matrix PlateMate* Liquid Handling Station. The membrane was then blocked with 0.5% Hammersten-grade casein in PBS. hSA was detected with rabbit anti-hSA antibody followed with alkaline phosphatase conjugated goat anti-rabbit IgG. Signal was generated by reaction with BCIP/NBT substrate allowing detection of as little as 0.5 pg hSA.

Part Number	Description	Pkg	Price	Qty
UltraBind Affinity Membrane
66544	20 x 20 cm sheets	10/pkg	323.45
66545	30 cm x 3 m roll	1/pkg	461.04

Contact Customer Service at 800-521-1520 for possible lead-time and ship date.