Nanosep® and Nanosep MF Centrifugal Devices

Simple, Reliable Concentrating and
Desalting of 50 to 500 µL Samples

• Ensures rapid processing of samples 
• Typical recoveries are > 90%
• Available with low proteinbinding Omega™, Bio-Inert®, and GHP membranes
• A wide range of MWCOs, color-coded for easy identification
• Constructed of low-binding polypropylene 
• Ultrasonically welded seals prevent bypass or seal failure
• Fits standard centrifuge rotors that accept 1.5 mL tubes

• Concentrate, purify, and desalt oligonucleotides, DNA, RNA, and proteins
• Clean up labeling and PCR reactions
• Isolate DNA from agarose gel slices
• Separate oligonucleotides and RNA from acrylamide gels
• Concentrate PCR products regardless of size with 30K device if primer removal is required

Materials of Construction

• Nanosep Devices
     Filter Media: Omega (modified polyethersulfone) ultrafiltration membrane
     Sample Reservoir, Membrane Support Base, and Filtrate Receiver: Polypropylene
• Nanosep MF Devices
     Filter Media: Bio-Inert (modified nylon) and GHP (hydrophilic polypropylene) membranes
     Sample Reservoir, Membrane Support Base, and Filtrate Receiver: Polypropylene

Effective Filtration Area

• 0.3 cm²

Dimensions

• Overall Length (Fully Assembled with Cap): 4.5 cm (1.8 in.)

Capacities

• Maximum Sample Volume: 500 µL
• Final Concentrate Volume: 15 µL
• Filtrate Receiver Volume: 500 µL
• Hold-up Volume (Membrane/Support): < 5 µL

Operating Temperature Range

• 0 - 40 °C (32 - 104 °F)

pH Range

• Nanosep Devices: 1 - 14
• Nanosep MF Devices: 3 - 14

Maximum Centrifugal Force

• 14,000 x g

Centrifuge

• Fits rotors that accept 1.5 mL tubes

Sanitization

• Provided non-sterile. May be sanitized by filtering 70% ethanol through the device prior to use.

• AcroWell™ 96-well Membrane-bottom Plates with GHP Membrane
• AcroWell™ 96-well Membrane-bottom Plates with BioTrace™ PVDF and NT Membranes 
• Acrodisc® Syringe Filters for General Aqueous Samples
• Acrodisc® Syringe Filters with GHP Membrane & GH Polypro (GHP) Membrane Disc Filters
• Acrodisc® Syringe Filters with Glass Fiber
• Acrodisc® Syringe Filters with HT Tuffryn® Membrane
• Acrodisc® Syringe Filters with Nylon Membrane & Nylaflo™ Membrane Disc Filters
• Acrodisc® Syringe Filters with PTFE Membrane & TF (PTFE) Membrane Disc Filters
• Acrodisc® Syringe Filters with PVDF Membrane & FP Vericel™ Membrane Disc Filters
• Acrodisc® Syringe Filters with Supor® Membrane
• Acrodisc® Syringe Filters with Versapor® Membrane
• Acrodisc® Unit with Mustang™ E Membrane
• Acrodisc® Unit with Mustang™ Q Membrane
• Acrodisc® Unit with Mustang™ S Membrane
• BioTrace™ NT Nitrocellulose Transfer Membrane
• BioTrace™ PVDF Transfer Membrane
• Biodyne® Transfer Membranes
• DMSO-Safe Acrodisc® Sterile Syringe Filter
• Ion Chromatography (IC) Acrodisc® Syringe Filters & Supor® (PES) Membrane Disc Filters
• UltraBind™ Affinity Membrane

DNA Recovery as a Function of Device MWCO

 

A 500 μL sample of a 100 μg/mL DNA fragment solution containing 50 and 500 bp double-stranded DNA fragments was centrifuged at 5,000 x g in Nanosep devices to a final volume of 50 μL. Recovered samples were quantitated using absorbance at 260 nm. The 100K device was able to differentiate between the sizes of the DNA fragments.

Omega™ Membrane Has the Lowest Non-Specific DNA Binding

A 500 μL sample of a 32P-labeled PCR product (400 bp, 50 ng/mL) was centrifuged at 5,000 x g in a 100K Nanosep or competitive device. The retained DNA was recovered in 40 μL TE (10 mM Tris, 1 mM EDTA, pH 8). The remaining radioactivity in the device was counted in a scintillation counter. A value at 1000 CPM roughly corresponds to 1% of the total radioactive sample. Omega membrane has the lowest non-specific binding, resulting in highest DNA recoveries. In general, DNA molecular weights do not correspond well with the MWCO because DNA is a long linear molecule. Effective retention of DNA by an ultrafiltration membrane requires a reduction in g-force to 5,000 x g. Otherwise, DNA can be forced through many MWCO membranes regardless of size.

Centrifugal Device Spin Times

Part Number	Description	Pkg	Price	Qty
Nanosep® Centrifugal Devices with Omega™ Membrane
OD003C33	3K, gray	24/pkg	79.28
OD003C34	3K, gray	100/pkg	268.85
OD003C35	3K, gray	500/pkg	1,128.44
OD010C33	10K, blue	24/pkg	79.28
OD010C34	10K, blue	100/pkg	268.85
OD010C35	10K, blue	500/pkg	1,131.03
OD030C33	30K, red	24/pkg	79.28
OD030C34	30K, red	100/pkg	268.85
OD030C35	30K, red	500/pkg	1,128.44
OD100C33	100K, clear	24/pkg	79.88
OD100C34	100K, clear	100/pkg	268.85
OD100C35	100K, clear	500/pkg	1,128.44
OD300C33	300K, orange	24/pkg	79.88
OD300C34	300K, orange	100/pkg	268.85
OD300C35	300K, orange	500/pkg	1,128.44
Nanosep MF Centrifugal Devices with Bio-Inert® Membrane
ODM02C33	0.2 µm, aqua	24/pkg	58.37
ODM02C34	0.2 µm, aqua	100/pkg	206.08
ODM02C35	0.2 µm, aqua	500/pkg	883.73
ODM45C33	0.45 µm, wildberry	24/pkg	58.37
ODM45C34	0.45 µm, wildberry	100/pkg	206.08
ODM45C35	0.45 µm, wildberry	500/pkg	883.73
Nanosep MF Centrifugal Devices with GHP Membrane
ODGHPC34	0.45 µm, clear	100/pkg	284.45
ODGHPC35	0.45 µm, clear	500/pkg	968.69

Contact Customer Service at 800-521-1520 for possible lead-time and ship date.