Capsules with HT Tuffryn® Membrane

Membrane Filters for Proven Applications

• Obtain accurate fills, uniform flow, and good throughput. Built-in air vent allows elimination of trapped air bubbles
• Integrity testable to ensure product and system integrity.
• Sterilization by gamma irradiation eliminates potential contamination by EtO residuals.

• Use for filtration of batches ranging from 1 to 10 liters (Micro Culture Capsule) and 10 to 20 liters (Culture, Maxi Culture, and Maxi Capsules) for the sterilization of buffers and cell culture media

Materials of Construction

• Filter Media: HT Tuffryn membrane (polysulfone)
• Housing and Filling Bell: Polycarbonate
• Vent Plug and Core: Polypropylene
• Filter Support: Polyester
• Sealing Materials: Polyurethane

Effective Filtration Area

• PN 12158: 300 cm²
• PN 12140 and 12170: 500 cm²
• PN 12141: 1290 cm²

Inlet/Outlet Connections

• PN 12140, 12170, and 12141: Hose barb fittings accept 12.7 mm (1/2 in.) ID tubing
• PN 12158: Stepped hose barbs 6.4 - 9.5 mm (1/4 - 3/8 in.) diameter

Typical Hold-Up Volume with 0.7 bar (70 kPa, 10 psi) air purge

• PN 12158: 15 mL
• PN 12140 and 12170: 25 mL
• PN 12141: 113 mL

Maximum Operating Temperature

• 100 °C (212 °F)

Maximum Operating Pressure

• 3.4 bar (340 kPa, 50 psi) at ambient temperature

Recommended Integrity Test Minimum Bubble Point – Water

• 2.4 bar (240 kPa, 35 psi)

Typical Water Flow Rate L/min at 0.7 bar (70 kPa, 10 psi)

• PN 12158: 1.2
• PN 12140, and 12170: 2.0
• PN 12141: 5.2

Bacterial Retention

• Lot samples retain a minimum of 10⁷ cfu/cm² of B. diminuta per modified ASTM F838, current revision

Endotoxin Level

• < 0.5 EU/mL using Limulus Amoebocyte Lysate (LAL) test

Biological Safety

• Passes United States Pharmacopeia (USP) Biological Reactivity Test, In Vivo <88>

Sterilization

• Sterilized by gamma irradiation
• If desired, autoclave once only prior to use at 121 - 123 °C (250 - 253 °F) for a maximum of 20 min

• HT Tuffryn® Membrane Disc Filters

Part Number	Description	Pkg	Price	Qty
Capsules with HT Tuffryn Membrane
12158	Micro Culture Capsule with filling bell, 0.2/0.2 µm, 300 cm2 (cell culture certified), gamma-irradiated	1/pkg	62.82
12140	Culture Capsule, 0.2/0.2 µm, 500 cm2 (cell culture certified), gamma-irradiated	1/pkg	95.63
12170	Culture Capsule with filling bell, 0.2/0.2 µm, 500 cm2 (cell culture certified), gamma-irradiated	1/pkg	97.52
12141	Maxi Culture Capsule, 0.2/0.2 µm, 1290 cm2 (cell culture certified), gamma-irradiated	1/pkg	137.65
Accessories and Replacement Parts
4227	Filling bell, sterile	5/pkg	71.47

Contact Customer Service at 800-521-1520 for possible lead-time and ship date.