BioTrace™ PVDF Transfer Membrane
Print Product Information
E-Mail
Download PDF
Ideally Suited for Western Transfers
with Total Protein Stain
- Low background with chemiluminescent detection systems
- Broad compatibility with commonly used solvents
- Ideal for protein sequencing
Media
- BioTrace PVDF (hydrophobic polyvinylidene fluoride)
Pore Size
- 0.45 µm
Typical Thickness
- 147 µm (5.8 mils)
Western Transfer to BioTrace PVDF Membrane
Serial dilutions of E. coli lysates were transferred from a 10 to 20% gradient gel to BioTrace PVDF and a competitive PVDF mem brane, then probed with rabbit anti-E. coli antibodies. Proteins were visualized using peroxi daseconjugated goat anti-rabbit anti bodies and 4-chloro-1-naphthol substrate solution.
Southern Transfer to BioTrace PVDF Membrane
Dilutions of λ-DNA HindIII fragments were separated electro phoretically on a 0.8% agarose gel and transferred under alkaline conditions to Pall Life Sciences BioTrace PVDF mem brane, as well as a competitive PVDF mem brane. The DNA was fixed by baking at 80 °C (176 °F) for 1 hour and the frag ments iden tified with a 32P λ-DNA probe hybridized at 65 °C (149 °F) for 16 hours.
Contact Customer Service at 800-521-1520 for possible lead-time and ship date.
|
Your shopping cart is empty. |
| *US List Price applies only to products delivered within the US. These prices do not reflect duties, taxes or tariffs, which may apply to purchases made outside of the US. See our General Limited Warranty. |