Biodyne® Nylon Transfer Membranes

High Sensitivity and Low Background
for Enhanced Detection and Resolution

• Will not crack, shrink, or tear when subjected to multiple cycles of hybridization, stripping, and reprobing
• Membranes are intrinsically hydrophilic for easy wetting
• Offers superior performance with radioactive (Biodyne B membrane) and non-radioactive (Biodyne A membrane) detection systems

Four Chemistries Provide Versatile Adsorption Properties

Biodyne A Membrane

• (Amphoteric Nylon 6,6) Membrane zeta potential can be modulated by changes in pH. Ideal for single probe or multiple rehybridizations, and applications where background is troublesome.

Biodyne B Membrane

• (Positively-charged Nylon 6,6) Pore surfaces are populated by a high density of quaternary ammonium groups. Our highest sensitivity nylon membrane for nucleic acid applications.

Biodyne C Membrane

• (Negatively-charged Nylon 6,6) Can be derivatized by coupling reactions through the carboxyl groups on the pore surfaces.

Biodyne Plus Membrane

• (Positively-charged Nylon 6,6 with an extremely high isoelectric point) With certain nonradioactive detection systems, it is more sensitive than Biodyne A membrane while exhibiting lower background than Biodyne B membrane.

Filter Media

• Biodyne A Membrane: Amphoteric Nylon 6,6
• Biodyne B and Plus Membranes: Positively-charged Nylon 6,6
• Biodyne C Membrane: Negativelycharged Nylon 6,6

Pore Size

• 0.2, 0.45, and 1.2 μm

Typical Thickness

Membrane	μm	mils
Biodyne A	5.5 - 7.0	139.7 - 177.8
Biodyne B	5.7 - 6.7	144.8 - 170.2
Biodyne Plus	5.7 - 6.7	144.8 - 170.2
Biodyne C	11.0 - 13.01	279.4 - 330.2

Solvent Compatibility

• Resistant to common solvents such as acetone, alcohol, chlorinated aliphatic hydrocarbons, formamide, 2M NaOH, DMSO, and dimethylformamide. Not compatible with concentrated formic acid (> 50%), HCl (> 4M), oxidizing agents, and long exposures (days to weeks) to pH < 2.

¹Dual layer measurements

Biodyne® B Membrane Withstands Multiple Cycles of Stripping and Reprobing

Lambda-HindIII fragments were separated in an agarose gel and transferred to Biodyne B membrane using the Pall Improved Alkaline Transfer. The blot was stripped completely and reprobed four times without loss of signal intensity. Bands were detected using a chemiluminescent detection system. 

1A - 4A: blot after (re)probing
1B - 4B: blot after stripping, prior to re(probing)

Fluorescent Detection of DNA Using Biodyne Plus Membrane

ng total DNA

Dilutions of HindIII-digested I-DNA (1000 - 1 ng/lane) were separated in an agarose gel and transferred to Biodyne Plus membrane. Signal was generated using a fluorescein-labeled probe, antifluorescein-alkaline phosphatase conjugate, and precipitating substrate. The image was generated by scanning the blot with a FluorImager* system. *FluorImager is a registered trademark of Molecular Dynamics.

Part Number	Description	Pkg	Price	Qty
Biodyne A Membrane
60113	0.2 µm, 30 cm x 3 m roll	1/pkg	385.74
60102	0.45 µm, 82 mm discs	50/pkg	192.94
60103	0.45 µm, 85 mm discs	50/pkg	199.43
60104	0.45 µm, 132 mm discs	50/pkg	337.57
60105	0.45 µm, 137 mm discs	50/pkg	352.96
60101	0.45 µm, 7 x 8.5 cm sheets	10/pkg	100.75
60100	0.45 µm, 20 x 20 cm sheets	10/pkg	296.60
60120	0.45 µm, 20 cm x 3 m roll	1/pkg	287.89
60106	0.45 µm, 30 cm x 3 m roll	1/pkg	385.74
60108	1.2 µm, 30 cm x 3 m roll	1/pkg	385.74
Biodyne B Membrane, 0.45 µm
60202	82 mm discs	50/pkg	192.94
60203	85 mm discs	50/pkg	199.43
60204	132 mm discs	50/pkg	337.57
60205	137 mm discs	50/pkg	352.96
60201	7 x 8.5 cm sheets	10/pkg	100.75
60200	20 x 20 cm sheets	10/pkg	293.70
60209	20 cm x 1 m roll	1/pkg	149.74
60208	20 cm x 3 m roll	1/pkg	287.89
60207	30 cm x 3 m roll	1/pkg	385.74
Biodyne C Membrane, 0.45 µm
60316	82 mm discs	50/pkg	192.94
60317	85 mm discs	50/pkg	199.43
60318	132 mm discs	50/pkg	339.20
60319	137 mm discs	50/pkg	352.96
60315	7 x 8.5 cm sheets	10/pkg	100.75
60314	20 x 20 cm sheets	10/pkg	293.70
Biodyne Plus Membrane, 0.45 µm
60402	82 mm discs	50/pkg	192.94
60403	85 mm discs	50/pkg	199.43
60404	132 mm discs	50/pkg	306.83
60405	137 mm discs	50/pkg	352.96
60401	7 x 8.5 cm sheets	10/pkg	100.75
60400	20 x 20 cm sheets	10/pkg	293.70
60406	30 cm x 3 m roll	1/pkg	385.74

Contact Customer Service at 800-521-1520 for possible lead-time and ship date.