Allows for Higher Sensitivity
and Shorter Assay Times
- Plate and membrane construction allow long incubations. Dual membrane construction allows support and protection of upstream layer. Downstream membrane also acts as a barrier to passive flow.
- White or opaque housing for use with fluorescent, luminescent, or radioactive detection systems.
- Low fluorescent background assures high signal-to-noise for fluorescent detection systems.
- 0.45 μm membrane retains cells, membrane compartments, or beads.
- Low binding membrane and housing material reduce time for passivation.
- Robotics-friendly design has rigid single-piece construction. Plates are stackable with or without lids.
- Minimal hold-up allows for greater detection accuracy.
- Membrane is sealed to bottom of plate using proprietary sealing technology that eliminates crosstalk.
- A serialized barcode label allows the use of automated tracking systems and identifies the membrane type.
AcroWell filter plates are designed for retention-based assays such as cell- or bead-based receptor:ligand screens.
Natural housing
- Delfia time-resolved fluorescence assays
- Fluorescent detection systems
White housing:
- Radiolabeled assays
- Luminescent detection
Materials of Construction
- Filter Media: GHP membrane (hydrophilic polypropylene)
- Membrane Support: Emflon® membrane (PTFE) backed with non-woven polypropylene
- Plate Housing: Polypropylene (natural or white opaque)
- Lid: Polystyrene
Dimensions
- Length: 12.8 cm (5.0 in.)
- Width: 8.6 cm (3.4 in.)
- Height With Lid: 1.7 cm (0.7 in.)
- Height Without Lid: 1.4 cm (0.6 in.)
Well-Bottom Area
Maximum Well Volume
Recommended Maximum Working Volume
Recommended Operating Vacuum
Maximum Vacuum
Typical Hold-Up Volume
Fluorescence Detection
- Fluorescence was detected using the Victor Multilabel Counter (PerkinElmer) using standard filters and settings for each plate.
Typical Background
|
| 5020 |
<25,000 |
<2,000 |
<2,0001 |
| 5021 |
<40,000 |
<40,000 |
<3,000 |
|
Light-Emitting Detection
- Luminometry, 20 pg of an alkaline phospatase-labeled antibody, was placed in the AcroWell™ filter plate and assayed using LumiGLO reagent. Adjacent wells were counted for light crosstalk and background.
GHP Membrane Exhibits Low Background Fluorescence
|
| Europium |
| 13 fmole |
53:1 |
60:1 |
8:1 |
6:1 |
| 6 |
27:1 |
30:1 |
4:1 |
3:1 |
| 3 |
14:1 |
16:1 |
3:1 |
2:1 |
| 2 |
8:1 |
10:1 |
2:1 |
1:1 |
| EU - Antibodies |
| 13 fmole |
62:1 |
114:1 |
9:1 |
13:1 |
| 6 |
31:1 |
60:1 |
5:1 |
7:1 |
| 3 |
16:1 |
33:1 |
3:1 |
4:1 |
| 2 |
8:1 |
16:1 |
2:1 |
3:1 |
| 1 |
4:1 |
6:1 |
2:1 |
2:1 |
|
The end result of having an extremely low background emission is that significantly lower concentrations of fluorescent label can be detected. We verified this attribute by comparing the ability of AcroWell 96 filter plates and competitive plates to detect the signal from serial dilutions of either Eu-labeled antibodies or Europium standard solution (PerkinElmer). The data indicates that the AcroWell 96 filter plate has signal-to-noise ratios consistent with ratios seen using a plain styrene plate. CPS = Counts Per Second. CN= Cellulose Nitrate.
GHP Membrane Permits Sensitive Detection Rivaling Radioactivity
Formerly, to achieve the highest sensitivities, radioactive ligands have been widely used for receptor binding assays. We show a direct comparison of a binding assay using [125I]-galanin or Eu3+-labeled galanin binding to GalR1- D98/Raji cell membranes (Valenzano et. al., 2000. Also refer to Pall Corporation Technical Report PN 33137.).Binding reactions were performed using 3% PEG-3350 and 5% DMSO in binding buffer. The fluorescent ligands were assayed using filtration techniques in an AcroWell 96 filter plate with GHP membrane in a natural polypropylene housing and detected with a Victor Multi-label Counter (PerkinElmer, Wallac) while equivalently treated and filtered radiolabeled ligands were resuspended in scintillent and counted in a MicroBeta* multilabel counter (PerkinElmer). Representative curves are shown with calculated Kd values (n=2). The signal to noise for the Eu-galanin is better than that measured for an equivalent radioactively labeled sample. CPS = Counts Per Second.
1Delfia enhancement solution added.
Print Product Information
E-Mail
Download PDF