Jumbosep™ Centrifugal Devices

Convenient and Reliable Concentration, Purification, and Diafiltration

• Concentration, purification, and diafiltration of 20 to 60 mL biological samples
• Typically concentrates 60 mL sample volumes to 5 mL in 30 minutes
• Provides high recoveries, typically > 90%
• Low protein-binding Omega™ membrane and polysulfone housing minimize losses due to non-specific binding
• Versatile Omega membrane is available in a variety of MWCOs, color-coded for easy identification
• Built-in deadstop prevents spinning to dryness
• Unique sealing mechanism prevents retentate leakage and filtrate contamination
• Economical - Sample reservoir and filtrate receiver can be sanitized or autoclaved, and reused

Replaces dialysis, chemical precipitation, and lyophilization in the following applications:

• Concentrating and desalting proteins
• Exchanging buffer or removing salt from chromatography eluates and gradient fractions
• Separating biomolecules from cell culture supernatants
• Concentrating or removing viruses
• Performing crude fractionation of protein mixtures
• Removing debris and particulates from cell lysates

Materials of Construction

• Filter Media: Omega (modified polyethersulfone) membrane
• Sample Reservoir and Filtrate Receiver: Polysulfone
• Sample Reservoir Cap: Polyethylene
• Insert Without Membrane: High density polyethylene
• Filtrate Receiver Cap and Insert Release: Polypropylene

Effective Filtration Area

• 15.2 cm²

Dimensions

• Outside Diameter (Maximum): 6 cm (2.4 in.)
• Overall Height (Fully Assembled with Cap): 11.3 cm (4.5 in.)

Capacities

• Maximum Sample Volume: 60 mL
• Final Concentrate Volume: 3.5 - 4 mL
• Maximum Filtrate Receiver Volume: 60 mL
• Hold-up Volume (membrane/support): 0.2 mL

Operating Temperature Range

• 0 - 40 °C (32 - 104 °F)

pH Range

• 1 - 14

Centrifuge

• Swinging bucket rotor is required that accepts flat-bottomed 250 mL bottles and is capable of spinning at up to 3,000 x g

Sanitization

• Provided non-sterile. The entire device, including the filter media, may be sanitized by filtering 70% ethanol through it prior to use. The sample reservoir and the filtrate receiver can be autoclaved. Do not autoclave the filter media.

• BioTrace™ NT Nitrocellulose Transfer Membrane
• BioTrace™ PVDF Transfer Membrane
• Biodyne® Transfer Membranes
• Macrosep® Advance Centrifugal Devices
• Macrosep® Centrifugal Devices
• Microsep™ Advance Centrifugal Devices
• Microsep™ & Microsep MF Centrifugal Devices
• Nanosep® & Nanosep MF Centrifugal Devices
• UltraBind™ Affinity Membrane

Jumbosep™ Device Reduces Processing Time Over Other Devices

1 mg/mL BSA solution was processed in each of the above devices until a 15-fold concentration is achieved.

 

Concentration Time

Concentrate dilute protein samples in less than 30 minutes with 10, 30, and 100K Jumbosep devices.

Part Number	Description	Pkg	Price	Qty
Jumbosep Centrifugal Device Starter Kits
FD000K65	Generic starter kit, (no membrane inserts)	4/pkg	149.65
FD003K65	3K starter kit, gray	4/pkg	199.59
FD010K65	10K starter kit, blue	4/pkg	199.59
FD030K65	30K starter kit, red	4/pkg	199.59
FD100K65	100K starter kit, clear	4/pkg	199.59
FD300K65	300K starter kit, orange	4/pkg	199.59
Jumbosep Centrifugal Device Membrane Inserts
OD003C65	3K membrane insert, gray	12/pkg	205.55
OD010C65	10K membrane insert, blue	12/pkg	205.55
OD030C65	30K membrane insert, red	12/pkg	205.55
OD100C65	100K membrane inser, clear	12/pkg	207.06
OD300C65	300K membrane insert, orange	12/pkg	207.06
Accessory Products
FD001X65	Filtrate receiver and cap	12/pkg	132.13
FD002X65	Sample reservoir and cap	12/pkg	178.44
FD003X65	Insert release	24/pkg	118.91

Contact Customer Service at 800-521-1520 for possible lead-time and ship date.