Envirochek® and Envirochek HV Sampling Capsules

For the concentration and recovery of Cryptosporidium oocysts and Giardia cysts from source or finished water

• Simple to use. No assembly or cleaning of filter holders or elution equipment.
• Saves time by allowing the processing multiple samples at the same time.
• Disposable design eliminates false positives from cross-contamination.
• Typically greater than 70% recovery of target organisms.
• Eliminates false negatives with 1 µm pore size membrane for 100% collection of Cryptosporidium and Giardia 
• Safer to use. Self-contained capsule mean that the filter element does not need to be handled or cut apart.
• Capsules are serialized for traceability. 
• Envirocheck HV capsules are 100% integrity tested.

Envirochek capsules are used for collection and recovery of Cryptosporidium oocysts and Giardia cysts in both finished and source water, including surface water, municipal water supplies, samples in containers, or wells.

• Envirochek capsules are validated and listed in U.S. EPA Methods 1622 and 1623, and used for sampling source water for Cryptosporidium and Giardia. Together, the EPA method and Envirochek capsule present a major improvement over the previous string wound cartridge method. The Envirochek capsule is also listed in ISO/DIS 15553.
• Envirochek HV capsules are designed for sampling up to 1,000 liters or more of treated water and validated for up to 50 liters of source water. The Envirochek HV incorporates a track etched membrane designed to process high volumes of treated water while maintaining high recovery characteristics (see Tables 1 and 2) and meeting U.S. EPA requirements. Envirochek HV capsules are also approved by the United Kingdom DWI standard operating protocols for monitoring drinking water for Cryptosporidium.

In the Envirochek sampling capsule method, the water sample to be tested is passed through the capsule either by sampling at the source or by taking a “grab” sample back to the laboratory and filtering it on the bench top. The capsule is then filled with an elution solution, placed on a laboratory shaker, and vigorously shaken to elute any captured oocysts and cysts. Up to eight capsules can be processed at once using Pall’s Laboratory Shaker. The elution solution is decanted and centrifuged to a pellet for further examination by the user’s method of choice.

Table 3 under the Performance tab shows the time savings from using the patented Envirochek sampling capsule and method. This simple process saves time and money by reducing steps and allowing the technician to attend to other tasks. The disposable capsule eliminates the arduous cleaning required by other products that use reusable filter housings.

Materials of Construction

Envirochek Capsule (PN 12110, 12107)  
Filter Media: Supor® membrane (hydrophilic polyethersulfone)
Housing: Polycarbonate
Filter Support Material: Polypropylene
End Caps: Green vinyl
Adhesive: Urethane

Envirochek HV Capsule (PN 12096, 12097, 12098, 12099)
Filter Media: Polyester, hydrophilic membrane
Housing: Polycarbonate
Filter Support Material: Polypropylene
End Caps: Blue vinyl
Adhesive: Urethane

Effective Filtration Area

1300 cm²

Dimensions

Length: 21.6 cm (8.5 in.)
Diameter: 6.1 cm (2.4 in.)

Inlet/Outlet Connections

12.7 mm (1/2 in.) straight hose barb

Elution Capacity

Minimum of 127 mL

Table 1IPR and MS/MSD Validation Data for Cryptosporidium Recovery Using Method 1622 and the Envirochek HV Capsule with 50 Liters Source Water Samples   

Sample Description	Lab	Sample Turbidity (ntu)	Spike Dose (#)	Mean % Rec.	RSD or RPD
IPR	1	< 0.1	95.9	44.9	9.5%
IPR	2	0.05	95.9	67.6	17.0
IPR	3	0.03	95.9	60.5	18.8
IPR Acceptable Range				24-100	< 55%
Matrix Blank	1	10.6	0
MS1	1	9.4	95.9
MS2	1	10.1	95.9	29.2	0.0%
Matrix Blank	2	2.1	0
MS1	2	2.1	95.9
MS2	2	2.1	95.9	72.0	20.3
Matrix Blank	3	1.8	0
MS1	3	1.8	95.9
MS2	3	1.8	95.9	56.3	11.1
IPR Acceptable Range				13-111	< 61%

IPR = Initial Precision and Recovery
MS/MSD = Matrix Spike/Matrix Spike Duplicate
RSD = Relative Standard Deviation
RPD = Relative Percent Difference

Table 2
IPR Tier 1 Validation Data for Cryptosporidium Recovery Using Method 1622 and the Envirochek HV Capsule for 1,000 Liters Finished Drinking Water Samples

Sample Description	Sample Turbidity (ntu)	Spike Dose (#)	% Rec.	Mean % Rec.	RSD or RPD
Reagent Blank	< 0.1	0
IPR2	< 0.1	99.3	68.5
IPR2	< 0.1	99.3	58.4
IPR3	< 0.1	99.3	60.4
IPR4	< 0.1	99.3	61.4	62.2	7.0
IPR Acceptable Range				13-143	< 67%

Table 3
Time Savings

	Approximate Time to Process Eight Samples (Minutes)
Step	Envirocheck Capsule	Other Marjor Protocol
Set-up	5	50
Elution	55	240
Concentration	65	240
Cleaning Equipment	0	320
Total Time	125	850
Time/Test (minutes)	16	106

Figure 1Envirochek HV Capsule Produces a Smaller Pellet Size When Sampling Turbid Water, Reducing Time and Cost for Analysis

A smaller pellet following concentration of the sample can reduce cost by eliminating the need for additional ISM kits and fewer slides. Testing time is reduced because additional slides do not have to be prepared and visually analyzed. The Envirochek HV capsule is designed to trap 100% of Cryptosporidium and Giardia while allowing finer particulate to pass through the filter that would add to pellet size. Each capsule is integrity tested to assure no bypass, providing highly accurate recovery rates as well as time and cost savings.

Laboratory Shaker 

 

For effective elution of Cryptosporidium oocysts and Giardia cysts off Envirochek sampling capsules

• Meets U.S. EPA requirements for Methods 1622 and 1623 for recovery of organisms.
• Processes up to eight Envirochek sampling capsules at one time.
• Accurate and consistent shaking speed with a digital read-out.
• Simulates human wrist action to save time, reduce fatigue, and provide consistent results.
• CE marked for European community use.
• UL-listed power cord also included.

Applications

• Specifically designed for use with Pall’s Envirochek sampling capsules for the elution of Cryptosporidium oocysts and Giardia cysts. Add an elution buffer to the capsule and the shaking action elutes the captured organisms off the membrane surface for further concentration and identification.
• Shaker accommodates up to eight Envirochek sampling capsules at one time.

Specifications

Timer
Includes 30-minute timer, calibrated at five minute intervals, or may be run continuously.

Shaking Speed
Variable speed regulation from 100 to 700 osc/minute (1,400 vibrations). Speed is monitored within 1 rpm of setpoint by digital tachometer.

Stroke
8 mm

Set-up
8 clamps included for attachment of Envirochek sampling capsules.

Type of Mounting
Benchtop. Suction cup feet prevent creeping.

Load Carrying Capacity
Accepts loads up to 4 kg (9 lb.)

Power Requirements
PN 4821: 115V, 50/60 Hz
PN 4822: 230V, 50/60 Hz
3-wire UL listed power cord

Power Consumption
80 watts

Dimensions
Height: 23 cm (9.1 in.)
Width: 77 cm (30.3 in.)
Depth: 27 cm (10.6 in.)
Weight: 11 kg (24.2 lb.) 

Laureth-12

Laureth-12 is a component required in the elution solution. Available in paste form, one 50-gram bottle per package. One bottle has sufficient volume for approximately 200 tests. For the complete formulation, refer to the Envirochek HV sampling capsule protocol, PN 33210. The formulation can also be found in U.S. EPA Methods 1622 and 1623.

Part Number	Description	Pkg	Price	Qty
Envirochek® and Envirochek HV Sampling Capsules
12099	Envirochek HV sampling capsule	1/pkg	110.51
12098	Envirochek HV sampling capsule, bulk pack, individually bagged	25/pkg	2,511.73
12110	Envirochek sampling capsule	1/pkg	108.16
12107	Envirochek sampling capsule, bulk pack, individually bagged	25/pkg	2,433.85
Accessory Products
4820	Laureth-12 Paste, 50 g bottle	1/pkg	42.89
4821	Laboratory Shaker, 115 V, 50/60 Hz	1/pkg	2,500.00
4822	Laboratory Shaker, 230 V, 50/60 Hz CE	1/pkg	2,500.00

Contact Customer Service at 800-521-1520 for possible lead-time and ship date.